MMP-12 ELISA Development Kit

Name: MMP-12 ELISA Development Kit
Brand: Novus Biologicals
SKU: DDXK-E-MMP12-1

Novus Biologicals, part of Bio-Techne | Catalog # DDXK-E-MMP12-1

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Sandwich ELISA

Assay Range

0.008 - 1 ug/ml

Sample Type

Human cell culture supernatants and biological fluids (plasma, serum, sputum) (100 uL)

Sensitivity

<50pg/ml

Reactivity

Human

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Product Specifications

Description

The human HME-MMP12 ELISA kit is for the quantitative determination of human HME-MMP12 in human cell culture supernatants, plasma, serum, and various biological fluids (sputum). This ELISA kit contains the specific components required for the development of human HMEMMP12 sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on 3 X 96-well plates. The DDX HME-MMP12 ELISA kit is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). The capture monoclonal antibody specific for HME-MMP12 is coated on a 96-well plate. Standards and samples are added to the wells, and any HME-MMP12 present binds to the immobilized antibody. The wells are washed and a HRPO-conjugated anti-HME-MMP12 monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. To produce color in proportion to the amount of HME-MMP12 present in the sample, TMB substrate solution is loaded and absorbance is measured at 620 nm.

Sample Volume Required

100 uL

Conjugate

Unconjugated

Application Notes

The human HME-MMP12 ELISA kit is for quantitative determination of HME-MMP12 concentrations in human cell culture supernatants and biological fluids (plasma, serum, sputum). This kit is for research purpose only.

Scientific Data Images for MMP-12 ELISA Development Kit

ELISA: MMP-12 ELISA Development Kit [DDXK-E-MMP12-1]

ELISA: MMP-12 ELISA Kit [DDXK-E-MMP12-1] - Specificity of the ELISA for MMP-12. Detection of serial dilution of recombinant MMP-12 protein by the ELISA. (Black blocks) No recombinant MMP-1 (triangle), MMP-3 (upside down triangle), or MMP-9 (open square)

Kit Contents for MMP-12 ELISA Development Kit

Capture Antibody: 0.5 mg/mL of mouse anti-HME-MMP12 monoclonal antibody (clone 705D10)
Detection Antibody: 0.5mg/mL of HRPO-conjugated anti-HME-MMP12 mouse monoclonal antibody (clone 706F9.01)
Standard: Each vial contains a solution of recombinant HME-MMP12 (concentration indicated on the vial) produced, purified and concentrated from transfected eukaryotic cells. A 8 point standard curve using 2-fold serial dilutions in sample dilution buffer

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-12

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. HME/MMP-12, also called metalloelastase, is critical for invasion and destruction in pathologies such as aneurysm and emphysema. HME mRNA and protein were previously described as expressed in human alveolar macrophages (Curci JA et al, 1998, J Clin Invest, 102(11):1900-10). The predicted molecular mass of the HME proenzyme is 54 kDa and readily undergoes NH2- and COOH-terminal processing to an active 45KDa form and a mature 22 kDa form. HME is a unique human metalloproteinase that displays elastolytic activity. We have isolated HME-MMP12 cDNA from a human CD34+-derived DC (GM-CSF+TNF) library. Expression analysis revealed HME-MMP12 expression in lung and placenta. Anti-HME-MMP12 monoclonal antibodies were obtained after mice immunization with HME-MMP12-transfected eukaryotic cells. These antibodies were used to set up a quantitative immunoassay for the detection of human HME-MMP12 (Demedts IK et al, 2006; Thorax, 61:196-201; Lapan et al, BMC Pulmonary Medicine, 2010, 10:40).

Long Name

Matrix Metalloproteinase 12

Alternate Names

MMP12

Gene Symbol