Mouse MMP-13 ELISA Kit (Colorimetric)

Name: Mouse MMP-13 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-06930

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06930

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse MMP-13 in samples. No significant cross-reactivity or interference between Mouse MMP-13 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.23

Inter-Assay Precision (Precision between assays) %CV < 5.22

Recovery for Mouse MMP-13 ELISA Kit (Colorimetric)

Recovery

89 - 105%

Linearity

Scientific Data Images for Mouse MMP-13 ELISA Kit (Colorimetric)

ELISA: Mouse MMP-13 ELISA Kit (Colorimetric) [NBP3-06930] -

ELISA: Mouse MMP-13 ELISA Kit (Colorimetric) [NBP3-06930] - Standard Curve Reference

Kit Contents for Mouse MMP-13 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-13

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.

Long Name

Matrix Metalloproteinase 13

Alternate Names

MMP13

Gene Symbol