Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

Mouse BAFF/BLyS/TNFSF13B ELISA Standard Curve

Detection of Mouse BAFF/BLyS/TNFSF13B by ELISA

OTULIN Deficiency in Myeloid Cells Leads to Neutrophil Infiltration, Multi-organ Inflammation, Hyper-Immunoglobulinemia, and Autoimmunity(A and B) Micrographs of H&E and Masson’s trichome stained sections of livers and spleens from 4- to 9-months-old LysMCre-Otulinflox mice reveal (A) infiltration in and fibrosis (right, arrowheads) of liver parenchyma and (B) distorted spleen architecture with germinal center activation (left, arrowheads) and fibrosis (right, arrowheads) in LysMCre-OtulinLacZ/flox mice. Scale bars, 200 μm. Micrographs are representative of five mice of each genotype from two independent experiments.(C and D) Flow cytometry analysis of total cellular infiltrate (CD45.1+ and CD45.2+) in peritoneal lavage (PL), spleen, liver, and kidney of LysMCre-Otulinflox mice presented as (C) representative dot plots of neutrophils with percent of cells in gate indicated and (D) total cell number of neutrophils, macrophages, and CD8+ T cells.(E) Concentrations of immunoglobulins in serum from 4- to 9-months-old LysMCre-Otulinflox mice.(F) ELISA analysis of serum autoantibody reactivity to ENA, dsDNA, and Smith antigen from 3- to 9-months-old LysMCre-Otulinflox mice.(G) ELISA analysis of serum B cell activating factor (BAFF) concentration from 3- to 9-months-old LysMCre-Otulinflox mice.(E–G) Data are mean of two technical replicas.(D–F) Data are presented as mean ± SEM, and n represents number of mice.See also Figure S5. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27523608), licensed under a CC-BY license. Not internally tested by R&D Systems.