Mouse CCL20/MIP-3 alpha Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MCC200
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), Heparin Plasma (50 µL)
Sensitivity
0.49 pg/mL
Assay Range
3.9-250 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Product Summary for Mouse CCL20/MIP-3 alpha Quantikine ELISA Kit
The Quantikine Mouse MIP-3 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MIP-3 alpha in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MIP-3 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MIP-3 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MIP-3 alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse MIP-3 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 23 | 23 | 23 |
| Mean (pg/mL) | 10 | 43 | 118 | 11 | 40 | 112 |
| Standard Deviation | 0.6 | 1.7 | 5.9 | 1.0 | 3.6 | 7.5 |
| CV% | 6.0 | 4.0 | 5.0 | 9.1 | 9.0 | 6.7 |
Recovery for Mouse CCL20/MIP-3 alpha Quantikine ELISA Kit
The recovery of mouse MIP-3 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 100 | 96-106 |
| Heparin Plasma (n=4) | 98 | 85-115 |
| Serum (n=4) | 106 | 95-119 |
Linearity
To assess the linearity of the assay, samples spiked with recombinant mouse MIP-3 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse CCL20/MIP-3 alpha Quantikine ELISA Kit
Mouse CCL20/MIP-3 alpha ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL20/MIP-3 alpha
Alternate Names
exodus-1, LARC, MIP-3 alpha, MIP3 alpha
Gene Symbol
CCL20
Additional CCL20/MIP-3 alpha Products
Product Documents for Mouse CCL20/MIP-3 alpha Quantikine ELISA Kit
Product Specific Notices for Mouse CCL20/MIP-3 alpha Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.