Mouse CD40 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MCCD40
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
3.7 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse CD40 Quantikine ELISA Kit
The Quantikine Mouse CD40 Immunoassay is a 4.5 hour solid phase ELISA designed to measure CD40 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse CD40 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural CD40 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse CD40.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse CD40
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 21.0 | 67.2 | 159 | 22.7 | 70.9 | 169 |
| Standard Deviation | 1.5 | 3.3 | 5.2 | 1.7 | 2.9 | 7.8 |
| CV% | 7.1 | 4.9 | 3.3 | 7.5 | 4.1 | 4.6 |
Recovery for Mouse CD40 Quantikine ELISA Kit
The recovery of mouse CD40 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 102 | 86-119 |
| EDTA Plasma (n=4) | 96 | 82-109 |
| Heparin Plasma (n=4) | 92 | 83-109 |
| Serum (n=4) | 92 | 81-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse CD40 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse CD40 Quantikine ELISA Kit
Mouse CD40/TNFRSF5 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD40/TNFRSF5
Alternate Names
CD40, TNFRSF5
Gene Symbol
CD40
Additional CD40/TNFRSF5 Products
Product Documents for Mouse CD40 Quantikine ELISA Kit
Product Specific Notices for Mouse CD40 Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.