Mouse EGF Quantikine ELISA Kit

Mouse EGF ELISA Cell Culture Supernate/Tissue Homogenate/ Urine Standard Curve

Mouse EGF ELISA Serum/Plasma Standard Curve

Detection of Mouse EGF by ELISA

STAT3 alters tumour microenvironment and angiogenesis.(a) IHC analysis of von Willebrand Factor (vWF) and CD31 is shown in consecutive sections of murine lung tumours of the indicated genotype. On right, CD31+ counts per tumour area (mm2) quantification at indicated time points. Data were analysed by Student’s t-test and displayed as mean±s.e.m. n≥5 tumours per mouse with n≥4 mice per genotype and time point. Scale bar, 50 μm. (b) ELISA of VEGFA levels in lung tumour lysates at indicated time points. Data were analysed by Student’s t-test and displayed as mean±s.d., n≥6 mice per genotype and time point. (c) Expression levels of Pdgfa in total lungs were measured by quantitative real-time PCR at indicated time points. Values are presented as fold change of relative mRNA expression compared with Stat3 deltaLep/ deltaLep:Kras+/+ mice. Data were analysed by one-way analysis of variance (ANOVA) with Tukey’s multiple comparison test and are shown as mean± s.d., n≥5 animals/genotype. (d) Flow cytometric analysis of Cd11b+Gr1+ granulocytes and Cd11b+ F4/80+ macrophages in bronchoalveolar lavage (BAL) at 6 and 13 weeks post AdCre. Data were analysed by one-way ANOVA with Tukey’s multiple comparison test and are shown as mean±s.e.m. (e) Flow cytometric analysis of myeloid-derived suppressor cell (MDSC) subsets at 6 and 13 weeks post AdCre. Data were analysed by one-way ANOVA with Tukey’s multiple comparison test and are shown as mean± s.e.m. (f) Ratio of CD4+/CD8+ T-cell counts in BAL are shown. Data were analysed by Kruskal–Wallis test with Dunn’s multiple comparison testing and are shown as mean±s.e.m. Data displayed in d–f are n≥6 mice per genotype and time point, 13-week group represents two independent experiments. (g) Flow cytometric analysis of Cd11b+Gr1+ and Cd11b+ F4/80+ cells of A549 shControl versus A549-shSTAT3 xenograft tumours. Data were analysed by Student’s t-test and are displayed as mean±s.e.m. (n≥8 tumours; ≥4 mice per group). (h) IHC analysis and representative pictures of CD31+ counts per xenograft tumour area (mm2). Scale bar, 100 μm (n=8 tumours/≥4 mice). Data were analysed by Student’s t-test and are shown as mean±s.e.m. For all graphs: *P<0.05; **P<0.01; ***P<0.001. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms7285), licensed under a CC-BY license. Not internally tested by R&D Systems.