Mouse G-CSF ELISA Kit - Quantikine
R&D Systems, part of Bio-Techne | Catalog # MCS00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (50 µL)
Sensitivity
5 pg/mL
Assay Range
14.1-900 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse G-CSF Quantikine ELISA Kit
The Quantikine Mouse G-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse G-CSF in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse G-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse G-CSF accurately. Results obtained using natural mouse G-CSF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse G-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 64.4 | 196 | 697 | 64.8 | 195 | 620 |
| Standard Deviation | 2.3 | 8.8 | 43 | 4.1 | 15.5 | 50.6 |
| CV% | 3.6 | 4.5 | 6.2 | 6.3 | 7.9 | 8.2 |
Recovery for Mouse G-CSF Quantikine ELISA Kit
The recovery of mouse G-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=7) | 92 | 82-110 |
| Serum (n=5) | 96 | 80-119 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse G-CSF in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse G-CSF Quantikine ELISA Kit
Mouse G-CSF ELISA Standard Curve
Detection of Mouse G-CSF by ELISA
Neutralization of TNF Ameliorates Inflammation Caused by OTULIN Deficiency(A–J) Measurements from tamoxifen (tx)-treated (arrows) CreERT2-Otulinflox bone marrow chimeras injected with (A–C) anti-TNF neutralizing antibodies ( alphaTNF) (data were pooled from two independent experiments), (D–F) anti-G-CSF-neutralizing antibodies ( alphaG-CSF), (G–I) anti-IL-6-neutralizing antibodies ( alphaIL-6), or isotype control as indicated.(A, D, and G) Body weight of CreERT2-Otulinflox chimeric mice treated with neutralizing antibodies as indicated.(B, E, and H) Blood neutrophil counts from CreERT2-Otulinflox chimeric mice treated as indicated.(C, F, and I) Total number of infiltrating CD11b+Gr-1+ neutrophils in spleen and peritoneal lavage (PL) measured by flow cytometry from CreERT2-Otulinflox chimeric mice treated as indicated.(J) Heatmap of Luminex multiplex analysis of cytokines and chemokines in serum from terminal bleeds on days 6 or 7 from chimeric mice treated as indicated. Numbers indicate relative change compared to isotype-treated del/flox mice within each experiment. G-CSF levels for alphaG-CSF and alphaIL-6 were measured by ELISA. Asterisks (∗) indicate the level of statistical significance.(K) Model of TNF-driven systemic inflammation and the contributions from different cytokines in OTULIN-deficient mice.(A–J) Data are presented as mean ± SEM, and n represents number of mice.See also Figure S3. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27523608), licensed under a CC-BY license. Not internally tested by R&D Systems.Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: G-CSF
Mouse G-CSF
receptor (G-CSF R) is a 120 kDa, type I transmembrane glycoprotein that belongs
to the hematopoietin receptor superfamily. The mature protein is 812 amino
acids in length and contains a 601 aa extracellular region, a 24 aa
transmembrane segment, and a 187 aa cytoplasmic domain. The extracellular
region contains multiple modules, including an N-terminal Ig-like domain, a
cytokine receptor homology domain, and three fibronectin type III domains.
Based on crystallographic study, G-CSF receptor forms a complex with the ligand
in a 2:2 ratio. Mouse and human G-CSF receptor share 63% aa sequence identity.
Cells known to express G-CSF R include monocytes and neutrophils,
megakaryocytes and platelets, CD34+ CD33+ and CD34+ CD38+ hematopoietic
progenitors, trophoblasts, endothelial cells and various tumor cell types.
G-CSF is an
important regulator for granulopoiesis in vivo. It has been demonstrated that
G-CSF can support the growth of multi-lineage hematopoietic progenitor cells
without influencing their commitment to the myeloid lineage and mobilize
hematopoietic progenitor cells from the bone marrow into the bloodstream. On
mature neutrophils, G-CSF may regulate neutrophil survival by controlling their
rate of apoptosis. G-CSF has also been shown to enhance the functional capacity
of mature neutrophils. As a consequence of its effects on hematopoietic
progenitor cells, G-CSF has been shown to enhance monocytopoiesis in the
presence M-CSF. Within the peripheral blood stem cell population mobilized with
G-CSF, selective increases in the number of T helper 2-inducing dendritic cells
are found.
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
Additional G-CSF Products
Product Documents for Mouse G-CSF Quantikine ELISA Kit
Product Specific Notices for Mouse G-CSF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.