Mouse GM-CSF Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MGM00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
5.8 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse GM-CSF Quantikine ELISA Kit
The Quantikine Mouse GM-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse GM-CSF in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse GM-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse GM-CSF accurately. Results obtained using natural mouse GM-CSF showed dose-response curves that were parallel to the standard curves obtained using the recombinant standards. These results indicate that this kit can be used to determine relative mass values for natural mouse GM-CSF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse GM-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 18.5 | 67.2 | 219 | 20.0 | 71.7 | 243 |
| Standard Deviation | 0.7 | 2.1 | 6.9 | 1.0 | 3.8 | 11.2 |
| CV% | 3.8 | 3.1 | 3.2 | 5.0 | 5.3 | 4.6 |
Recovery for Mouse GM-CSF Quantikine ELISA Kit
The recovery of mouse GM-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=8) | 96 | 82-114 |
| Serum (n=7) | 94 | 82-112 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse GM-CSF in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse GM-CSF Quantikine ELISA Kit
Mouse GM-CSF ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: GM-CSF
Long Name
Granulocyte Macrophage Growth Factor
Alternate Names
CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim
Entrez Gene IDs
Gene Symbol
CSF2
Additional GM-CSF Products
Product Documents for Mouse GM-CSF Quantikine ELISA Kit
Product Specific Notices for Mouse GM-CSF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.