Mouse Haptoglobin DuoSet ELISA

Name: Mouse Haptoglobin DuoSet ELISA
Brand: R&D Systems
SKU: DY4409-05

R&D Systems, part of Bio-Techne | Catalog # DY4409-05

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

62.5-4000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse

Mouse Haptoglobin DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all Haptoglobin ELISA Kits »

Product Summary for Mouse Haptoglobin DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Haptoglobin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Mouse Haptoglobin DuoSet ELISA

Mouse Haptoglobin ELISA Standard Curve

Kit Contents for Mouse Haptoglobin DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Haptoglobin

Haptoglobin is an acute phase glycoprotein that is an inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and binds with high affinity to circulating Hemoglobin. Association of Haptoglobin with free Hemoglobin protects tissues from Hemoglobin-mediated oxidative damage and facilitates Hemoglobin clearance. In addition, Haptoglobin has anti-inflammatory properties that are independent of Hemoglobin binding. It inhibits prostaglandin synthesis, scavenges nitric oxide, dampens the inflammatory response to endotoxins, and inhibits T cell proliferation and neutrophil respiratory bursts. Deletion of Haptoglobin in mice facilitates the development of autoimmune inflammation.