Mouse IFN-alpha All Subtype Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MFNAS0
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
3.79 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse IFN-alpha All Subtype Quantikine ELISA Kit
The Quantikine™ Mouse IFN-alpha All Subtype immunoassay is a 3.5 hour solid-phase ELISA designed to measure IFN-alpha in mouse cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant mouse IFN-alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IFN-alpha showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-alpha.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using one lot of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 82.2 | 590 | 199 | 79.3 | 602 | 195 |
| Standard Deviation | 3.04 | 15.9 | 3.08 | 5.11 | 57.4 | 7.93 |
| CV% | 3.7 | 2.7 | 1.5 | 6.4 | 9.5 | 4.1 |
Recovery for Mouse IFN-alpha All Subtype Quantikine ELISA Kit
The recovery of mouse IFN-alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 91-106 |
| EDTA Plasma (n=4) | 105 | 91-121 |
| Heparin Plasma (n=4) | 106 | 96-120 |
| Serum (n=4) | 102 | 86-118 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IFN-alpha were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse IFN-alpha All Subtype Quantikine ELISA Kit
Mouse IFN-alpha All Subtype Quantikine Natural Linearity Competitor Comparison
IFN-alpha samples containing a high concentration in various matrices are diluted with appropriate Calibrator Diluent to produce sample values within the dynamic range of the assay. R&D Systems natural linearity is between 98%-103% compared to 125%-180% for the top competitor.
Mouse IFN-alpha All Subtype Quantikine ELISA Spiked Linearity Competitor Comparison
IFN-alpha is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. R&D Systems linearity is between 88%-92% compared to 132%-201% for the top competitor.
Mouse IFN-alpha All Subtype Quantikine ELISA Spiked Recovery
IFN-alpha is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. R&D Systems recovery is 99%.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-alpha
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Mouse IFN-alpha All Subtype Quantikine ELISA Kit
Product Specific Notices for Mouse IFN-alpha All Subtype Quantikine ELISA Kit
For research use only
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