Mouse IFN-gamma ELISA Kit - Quantikine
R&D Systems, part of Bio-Techne | Catalog # MIF00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
2 pg/mL
Assay Range
9.4-600 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse IFN-gamma Quantikine ELISA Kit
The Quantikine Mouse IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-gamma in cell culture supernates and mouse serum. It contains E. coli-expressed mouse IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse IFN-gamma accurately. Results obtained using natural mouse IFN-gamma showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-gamma.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IFN-gamma
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum - Forty individual mouse serum samples were evaluated for the presence of mouse
IFN-gamma in this assay. Thirty-eight samples measured less than the lowest Mouse IFN-g Standard,
9.4 pg/mL. Two samples read 24 pg/mL and 60 pg/mL, respectively.
Cell Culture Supernates: Mouse splenocytes (2 x 106
cells/mL) were cultured for 3 days in RPMI plus 10% fetal bovine
serum and stimulated with 10 μg/mL PHA. An aliquot of the cell culture supernate was
removed, assayed for mouse IFN-gamma, and measured 32 ng/mL.
EL-4 mouse lymphoblast cells (9 x 105
cells/mL) were cultured for 2 days in DMEM plus
10% fetal bovine serum and stimulated with 10 μg/mL PHA and 10 ng/mL PMA. An aliquot of
the cell culture supernate was removed, assayed for mouse IFN-gamma, and measured 4 ng/mL.
Mouse lung conditioned media (1 lung, 1-2 mm pieces in 10 mL of medium) was collected
after culturing for 5 days in RPMI plus 10% fetal bovine serum. An aliquot of the cell culture
supernate was removed, assayed for mouse IFN-gamma, and measured 198 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 44.8 | 156 | 518 | 43.3 | 144 | 509 |
| Standard Deviation | 2.2 | 5.0 | 14.2 | 3.6 | 14.7 | 48.6 |
| CV% | 4.9 | 3.2 | 2.7 | 8.3 | 10.2 | 9.5 |
Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 45.1 | 143 | 528 | 42.8 | 139 | 518 |
| Standard Deviation | 2.1 | 4.4 | 11.8 | 3.6 | 13.6 | 46.5 |
| CV% | 4.7 | 3.1 | 2.2 | 8.4 | 9.8 | 9.0 |
Recovery for Mouse IFN-gamma Quantikine ELISA Kit
The recovery of mouse IFN-gamma spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=7) | 105 | 98-115 |
| Serum (n=5) | 97 | 91-106 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IFN-gamma in each matrix were diluted with the appropriate Calibrator Diluent and then assayed.
Scientific Data Images for Mouse IFN-gamma Quantikine ELISA Kit
Mouse IFN-gamma ELISA Calibrator Diluent RD5Y Standard Curve
Mouse IFN-gamma ELISA Calibrator Diluent RD6-12 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-gamma
Long Name
Interferon gamma
Alternate Names
IFG, IFI, IFNG, IFNgamma
Entrez Gene IDs
Gene Symbol
IFNG
Additional IFN-gamma Products
Product Documents for Mouse IFN-gamma Quantikine ELISA Kit
Product Specific Notices for Mouse IFN-gamma Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.