Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MOP00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
Sensitivity
6.9 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Product Summary for Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit
The Quantikine Mouse OPG Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse OPG in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse OPG and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse OPG accurately. Results obtained using natural mouse OPG showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse OPG.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse OPG
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 69.9 | 311 | 650 | 68.0 | 332 | 741 |
| Standard Deviation | 5.5 | 13.4 | 35.6 | 5.0 | 23.0 | 54.0 |
| CV% | 7.9 | 4.3 | 5.5 | 7.4 | 6.9 | 7.3 |
Recovery for Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit
The recovery of mouse OPG spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=9) | 101 | 85-116 |
| EDTA Plasma (n=9) | 97 | 88-107 |
| Serum (n=11) | 93 | 83-108 |
Linearity
To assess the linearity of the assay, five or more samples containing various concentrations of OPG in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit
Mouse Osteoprotegerin/TNFRSF11B ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Osteoprotegerin/TNFRSF11B
Additional Osteoprotegerin/TNFRSF11B Products
Product Documents for Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit
Product Specific Notices for Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.