Mouse PlGF-2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MP200
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL)
Sensitivity
1.84 pg/mL
Assay Range
23.4-1500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Product Summary for Mouse PlGF-2 Quantikine ELISA Kit
The Quantikine Mouse PlGF-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse PlGF-2 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse PlGF-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PlGF-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PlGF-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse PlGF-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 58 | 136 | 470 | 63 | 155 | 493 |
| Standard Deviation | 4.6 | 7.6 | 14 | 6.0 | 15 | 43 |
| CV% | 7.9 | 5.6 | 3.0 | 9.5 | 9.7 | 8.7 |
Recovery for Mouse PlGF-2 Quantikine ELISA Kit
The recovery of mouse PlGF-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 97 | 84-119 |
| EDTA Plasma (n=6) | 94 | 88-104 |
| Serum (n=6) | 90 | 81-102 |
Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse PlGF-2 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse PlGF-2 Quantikine ELISA Kit
Mouse PlGF-2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PlGF-2
Long Name
Placenta Growth Factor 2
Alternate Names
PlGF2
Gene Symbol
PGF
Additional PlGF-2 Products
Product Documents for Mouse PlGF-2 Quantikine ELISA Kit
Product Specific Notices for Mouse PlGF-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.