Mouse PRDC/GREM2 DuoSet ELISA

Name: Mouse PRDC/GREM2 DuoSet ELISA
Brand: R&D Systems
SKU: DY2069

R&D Systems, part of Bio-Techne | Catalog # DY2069

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

31.2-2000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse

Mouse PRDC/GREM2 DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all PRDC/GREM2 ELISA Kits »

Product Summary for Mouse PRDC/GREM2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse PRDC/GREM2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Mouse PRDC/GREM2 DuoSet ELISA

Mouse PRDC / GREM2 ELISA Standard Curve

Kit Contents for Mouse PRDC/GREM2 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween^® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PRDC/GREM2

PRDC (protein related to DAN and Cerberus) is a secreted, cysteine knot-containing BMP antagonist belonging to the Cerberus/DAN (CAN) family. Mammalian CAN family members, including Gremlin, DAN, Cerberus, COCO, SOST, and USAG-1, have the conserved 6 cysteine residues that form a cysteine knot, and two additional cysteine residues located in the cysteine knot loops that form an additional intrasubunit disulfide bond. Some members of this family, including PRDC, have an additional cysteine residue used for dimerization.

Long Name

Protein Related to DAN and Cerberus/Gremlin-2

Alternate Names

CKTSF1B2, DAND3, GREM2

Entrez Gene IDs

64388 (Human); 23893 (Mouse)

Gene Symbol

GREM2

Additional PRDC/GREM2 Products

Product Documents for Mouse PRDC/GREM2 DuoSet ELISA

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse PRDC/GREM2 DuoSet ELISA

For research use only

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