Mouse RAGE Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MRG00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
Sensitivity
4.81 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Mouse RAGE Quantikine ELISA Kit
The Quantikine Mouse RAGE Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse RAGE levels in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse RAGE and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse RAGE accurately. Results obtained using natural mouse RAGE showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse RAGE.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse RAGE.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Lysates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 66.1 | 236 | 518 | 68.8 | 242 | 559 |
| Standard Deviation | 5.02 | 14.5 | 24.3 | 5.44 | 13.4 | 32.6 |
| CV% | 7.6 | 6.1 | 4.7 | 7.9 | 5.5 | 5.8 |
Recovery for Mouse RAGE Quantikine ELISA Kit
The recovery of mouse RAGE spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 108 | 94-120 |
| EDTA Plasma (n=4) | 101 | 87-118 |
| Heparin Plasma (n=4) | 111 | 96-120 |
| Serum (n=4) | 107 | 90-117 |
| Tissue Lysates (n=4) | 103 | 91-120 |
| Urine (n=4) | 109 | 96-120 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse RAGE were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Mouse RAGE Quantikine ELISA Kit
Mouse RAGE ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: RAGE/AGER
To view our complete solutions for RAGE research, visit bio-techne.com.
Long Name
Receptor for Advanced Glycation End Products
Alternate Names
AGER, SCARJ1
Gene Symbol
AGER
Additional RAGE/AGER Products
Product Documents for Mouse RAGE Quantikine ELISA Kit
Product Specific Notices for Mouse RAGE Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.