Mouse/Rat cAMP Parameter Assay Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # KGE012B
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Tissue Homogenates (50 µL), EDTA Plasma (50 µL), Urine (10 µL)
Sensitivity
1.54 nmol/L
Assay Range
3.75-240 nmol/L (Cell Culture Supernates, Cell Lysates, Tissue Homogenates, EDTA Plasma, Urine)
Product Summary for Mouse/Rat cAMP Parameter Assay Kit
The R&D Systems cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, tissue homogenates, plasma, and urine.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Mouse, Rat
Specificity
Cyclic AMP in mouse and rat samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Tissue Homogenates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (nmol/L) | 27.7 | 58.7 | 96.9 | 28.2 | 61.7 | 102 |
| Standard Deviation | 0.5 | 1.0 | 3.9 | 2.2 | 4.8 | 6.5 |
| CV% | 1.8 | 1.7 | 4.0 | 7.8 | 7.8 | 6.4 |
Recovery for Mouse/Rat cAMP Parameter Assay Kit
The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 100 | 98-103 |
| Cell Lysates (n=2) | 106 | 106-107 |
| Mouse EDTA Plasma (n=4) | 97 | 92-101 |
| Rat EDTA Plasma (n=4) | 104 | 101-108 |
| Tissue Homogenates (n=4) | 110 | 105-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse/Rat cAMP Parameter Assay Kit
Mouse/Rat cAMP ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: cAMP
Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.
Long Name
Cyclic AMP
Alternate Names
Cyclic AMP
Additional cAMP Products
Product Documents for Mouse/Rat cAMP Parameter Assay Kit
Product Specific Notices for Mouse/Rat cAMP Parameter Assay Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.