Mouse/Rat IGF-I/IGF-1 ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # MG100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Tissue Homogenates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
8.4 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit
The Quantikine Mouse/Rat IGF-I Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IGF-I in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse IGF-I and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse and rat IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat IGF-I.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat IGF-I
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Individual mouse and rat serum and plasma samples were evaluated for
detectable levels of mouse and rat IGF-1 in this assay. Serum and plasma samples were not
matched.
Cell Culture Supernates:
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Mouse serum (n=20) | 742 | 513-924 | 113 |
| Mouse EDTA plasma (n=20) | 593 | 346-806 | 130 |
| Mouse heparin plasma (n=20) | 821 | 542-1447 | 203 |
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Rat serum (n=20) | 1158 | 436-1908 | 376 |
| Rat EDTA plasma (n=20) | 1016 | 430-2207 | 377 |
| Rat heparin plasma (n=20) | 1012 | 723-1444 | 208 |
Cell Culture Supernates:
J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 106
cells/mL) were cultured for
3 days in DMEM containing 10% fetal bovine serum and stimulated with 2.5 ng/mL LPS. The
cell culture supernate was assayed for mouse IGF-1 and measured 5842 pg/mL.
WEHI-3 mouse myelomonocytic leukemia cells were cultured for 2-4 days in RPMI
supplemented with 10% fetal bovine serum. The cell culture supernate was assayed for mouse
IGF-1 and measured 4944 pg/mL.
Rat lung conditioned media (1 lung, 1-2 mm pieces) was cultured for 2 days in 50 mL of DMEM
supplemented with 10% fetal bovine serum and stimulated with 100 ng/mL LPS and 50 ng/mL
PMA. The cell culture supernate was assayed for rat IGF-1 and measured 1514 pg/mL.
Rat heart conditioned media (1 heart, 1-2 mm pieces) was cultured for 4 days in 30 mL of
RPMI, supplemented with 10% fetal bovine serum, and stimulated with 2.5 ng/mL LPS. The
cell culture supernate was assayed for rat IGF-1 and measured 2888 pg/mL.
Tissue Homogenates:
Homogenates from mouse lung and mouse heart tissue were assayed for levels of mouse IGF-1
and measured 5862 pg/mL and 3158 pg/mL, respectively.
Homogenates from rat lung and rat heart tissue were assayed for levels of rat IGF-1 and
measured 5476 pg/mL and 1785 pg/mL, respectively.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 35 | 35 | 35 |
| Mean (pg/mL) | 82 | 269 | 921 | 86 | 256 | 863 |
| Standard Deviation | 4.6 | 8.9 | 37.7 | 7.8 | 11.8 | 37.4 |
| CV% | 5.6 | 3.3 | 4.1 | 9.1 | 4.6 | 4.3 |
Recovery for Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit
The recovery of mouse/rat IGF-I spiked to three levels throughout the range of the assay in the cell culture supernate samples was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=7) | 98 | 89-111 |
| Rat Cell Culture Supernates (n=6) | 98 | 82-108 |
Linearity
To assess the linearity of the assay, samples containing mouse or rat IGF-I in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit
Mouse/Rat IGF-I ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-I/IGF-1
Mature mouse IGF-1 is a non-glycosylated, 70 amino acid (aa)
secreted polypeptide that is derived from either a 153 aa or a 159 aa
preproprotein. It shares 99% and 94% aa sequence identity with rat and human
IGF-1, respectively. IGF-1 is synthesized in the liver and other tissues. It is
found in blood and other body fluids as a complex with specific high affinity
IGF binding proteins (IGFBP-1 to -6). The IGFBPs are expressed in specific
patterns during development. They are modulators of IGF actions, which control
IGF bioavailability to specific cell-surface receptors. Their functions are
further regulated by IGFBP proteases, which proteolytically cleave the IGFBPs
to lower the affinity with which they bind IGFs and increase IGF
bioavailability. Some IGFBPs also have IGF-Independent effects on cell
functions. IGF-Independent circulates primarily as a ternary complex with
IGFBP-3 or IGFBP-5 and the acid-labile subunit (ALS). Some IGF-1 is also
present in binary complexes with other IGFBPs. Whereas the ternary complexes
are generally restricted to the vasculature, the binary complexes freely enter
the tissues.
IGF-1 actions are mediated by two ubiquitously expressed
receptor tyrosine kinases: IGF-I R and Insulin R/CD220. IGF-I R and Insulin R
are disulfide-linked heterotetrameric complexes that consist of two alpha and
two beta subunits. For both of these receptors, the prepro proteins are cleaved
to produce extracellular alpha subunits which contain a cysteine-rich region
and ligand-binding fibronectin type III (FN-III) domains, and beta subunits
which contain an extracellular FN-III domain, transmembrane, and cytoplasmic
tyrosine kinase domains. A hybrid complex containing one IGF-I R and one
Insulin R also serves as a functional high affinity receptor for IGF-1. IGF-I
R-Insulin R hybrids respond primarily to IGF-1, potentially downregulating the
cellular response to Insulin. IGF signaling is also modulated by IGF binding
proteins and the scavenger receptor, IGF-I I R.
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
Additional IGF-I/IGF-1 Products
Product Documents for Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.