Mouse/Rat RGM-C/Hemojuvelin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MRGMC0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)
Sensitivity
4.46 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse/Rat RGM-C/Hemojuvelin Quantikine ELISA Kit
The Quantikine Mouse/Rat RGM-C/Hemojuvelin Immunoassay is a 4.5 hour solid phase
ELISA designed to measure mouse and rat RGM-C in cell culture
supernates, tissue homogenates, serum, and plasma. It contains
NS0-expressed recombinant mouse RGM-C and antibodies raised against the
recombinant factor. This immunoassay has been shown to quantitate the
recombinant factor accurately. Results obtained using natural RGM-C
showed dose-response curves that were parallel to the standard curves
obtained using the recombinant kit standards. These results indicate
that this kit can be used to determine relative mass values for natural
RGM-C.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Recombinant and natural mouse and rat RGM-C/Hemojuvelin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 75 | 244 | 751 | 72.8 | 228 | 726 |
| Standard Deviation | 3.60 | 6.10 | 23.0 | 5.35 | 16.6 | 47.8 |
| CV% | 4.8 | 2.5 | 3.1 | 7.3 | 7.3 | 6.6 |
Recovery for Mouse/Rat RGM-C/Hemojuvelin Quantikine ELISA Kit
The recovery of mouse/rat RGM-C spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 98 | 85-110 |
| EDTA Plasma (n=4) | 100 | 80-120 |
| Heparin Plasma (n=4) | 100 | 83-116 |
| Serum (n=4) | 105 | 88-120 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of RGM-C were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse/Rat RGM-C/Hemojuvelin Quantikine ELISA Kit
Mouse/Rat RGM-C/Hemojuvelin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: RGM-C/Hemojuvelin
Long Name
Repulsive Guidance Molecule C
Alternate Names
DL-M, Hemojuvelin, HFE2, HJV, JH, RGMC
Gene Symbol
HJV
Additional RGM-C/Hemojuvelin Products
Product Documents for Mouse/Rat RGM-C/Hemojuvelin Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat RGM-C/Hemojuvelin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.