Mouse/Rat TREM-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MTRM10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
5.4 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse/Rat TREM-1 Quantikine ELISA Kit
The Quantikine Mouse/Rat TREM-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse or rat TREM-1 in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse TREM-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural TREM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring TREM-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat TREM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 26 | 26 | 26 |
| Mean (pg/mL) | 79.8 | 248 | 1222 | 79.3 | 227 | 1160 |
| Standard Deviation | 4.5 | 22.0 | 51.6 | 6.3 | 14.0 | 62.2 |
| CV% | 5.6 | 8.9 | 4.2 | 7.9 | 6.2 | 5.4 |
Recovery for Mouse/Rat TREM-1 Quantikine ELISA Kit
The recovery of TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=5) | 98 | 87-109 |
| Mouse EDTA Plasma (n=6) | 99 | 90-108 |
| Mouse Heparin Plasma (n=5) | 101 | 89-110 |
| Mouse Serum (n=5) | 98 | 81-106 |
| Mouse Tissue Homogenates (n=2) | 100 | 91-104 |
| Rat Cell Culture Supernates (n=5) | 97 | 90-109 |
| Rat EDTA Plasma (n=5) | 100 | 85-112 |
| Rat Heparin Plasma (n=5) | 99 | 85-113 |
| Rat Serum (n=4) | 107 | 84-120 |
| Rat Tissue Homogenates (n=2) | 99 | 95-103 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TREM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse/Rat TREM-1 Quantikine ELISA Kit
Mouse/Rat TREM-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TREM-1
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Alternate Names
CD354, TREM1
Gene Symbol
TREM1
Additional TREM-1 Products
Product Documents for Mouse/Rat TREM-1 Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat TREM-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.