Mouse SP-D Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MSFPD0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
9.43 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse SP-D Quantikine ELISA Kit
The Quantikine Mouse SP-D immunoassay is a 4.5 hour solid-phase ELISA designed to measure SP-D in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant mouse SP-D and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse SP-D. Results obtained using natural mouse SP-D showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse SP-D.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse SP-D.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 4 | 4 | 4 | 4 | 4 | 4 |
| Mean (pg/mL) | 158 | 534 | 1270 | 169 | 531 | 1267 |
| Standard Deviation | 5.75 | 13.8 | 43.0 | 14.0 | 34.4 | 120 |
| CV% | 3.6 | 2.6 | 3.4 | 8.3 | 6.5 | 9.5 |
Recovery for Mouse SP-D Quantikine ELISA Kit
The recovery of mouse SP-D spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 92-105 |
| EDTA Plasma (n=4) | 97 | 90-103 |
| Heparin Plasma (n=4) | 97 | 84-104 |
| Serum (n=4) | 96 | 84-109 |
| Tissue Lysates (n=4) | 101 | 91-113 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SP-D in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay
Scientific Data Images for Mouse SP-D Quantikine ELISA Kit
Mouse SP-D ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SP-D
Long Name
Surfactant Pulmonary Associated Protein D
Alternate Names
Collectin 7, PSP-D, SFTP4, SFTPD, SPD
Gene Symbol
SFTPD
Additional SP-D Products
Product Documents for Mouse SP-D Quantikine ELISA Kit
Product Specific Notices for Mouse SP-D Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.