Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MTR00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL)
Sensitivity
5 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
The Quantikine Mouse TRANCE Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TRANCE in cell culture supernates and serum. It contains NS0-expressed recombinant mouse TRANCE and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse TRANCE accurately. Results obtained using natural mouse TRANCE showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TRANCE.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Free natural and recombinant mouse TRANCE
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 99 | 261 | 830 | 111 | 285 | 886 |
| Standard Deviation | 8.0 | 6.8 | 18.3 | 8.8 | 17.8 | 57.8 |
| CV% | 8.1 | 2.6 | 2.2 | 7.9 | 6.2 | 6.5 |
Recovery for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
The recovery of mouse TRANCE spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 99 | 87-116 |
| Serum (n=6) | 93 | 80-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with mouse TRANCE were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
Mouse TRANCE/TNFSF11/RANK L ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TRANCE/TNFSF11/RANK L
Long Name
TNF-related Activation-induced Cytokine
Alternate Names
CD254, ODF, OPGL, RANK L, RANKL, TNFSF11
Gene Symbol
TNFSF11
Additional TRANCE/TNFSF11/RANK L Products
Product Documents for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
Product Specific Notices for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.