Mouse VCAM-1/CD106 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MVC00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
Sensitivity
0.06 ng/mL
Assay Range
0.31-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Product Summary for Mouse VCAM-1/CD106 Quantikine ELISA Kit
The Quantikine Mouse soluble VCAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sVCAM-1 levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sVCAM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sVCAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sVCAM-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse sVCAM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.96 | 2.84 | 8.55 | 0.87 | 2.64 | 8.50 |
| Standard Deviation | 0.05 | 0.13 | 0.37 | 0.07 | 0.18 | 0.63 |
| CV% | 5.2 | 4.6 | 4.3 | 8.0 | 6.8 | 7.4 |
EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.93 | 2.61 | 8.58 | 0.86 | 2.46 | 8.46 |
| Standard Deviation | 0.07 | 0.17 | 0.40 | 0.05 | 0.11 | 0.65 |
| CV% | 7.5 | 6.5 | 4.7 | 5.8 | 4.5 | 7.7 |
Recovery for Mouse VCAM-1/CD106 Quantikine ELISA Kit
The recovery of mouse sVCAM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 100 | 89-112 |
| EDTA Plasma (n=4) | 100 | 81-119 |
| Serum (n=10) | 99 | 80-118 |
Linearity
To assess the linearity of the assay, six samples of each matrix containing and/or spiked with high concentrations of mouse sVCAM-1 were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse VCAM-1/CD106 Quantikine ELISA Kit
Mouse VCAM-1/CD106 ELISA Cell Culture Supernate Standard Curve
Mouse VCAM-1/CD106 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VCAM-1/CD106
Long Name
Vascular Cell Adhesion Molecule 1
Alternate Names
CD106, VCAM1
Gene Symbol
VCAM1
Additional VCAM-1/CD106 Products
Product Documents for Mouse VCAM-1/CD106 Quantikine ELISA Kit
Product Specific Notices for Mouse VCAM-1/CD106 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.