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Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81154

Novus Biologicals, part of Bio-Techne
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NBP2-81154

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Muscarinic Acetylcholine Receptor M1/CHRM1 in samples. No significant cross-reactivity or interference between Human Muscarinic Acetylcholine Receptor M1/CHRM1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.11%

Inter-Assay Precision (Precision between assays) CV% < 5.49%

Recovery

Recovery

86-105%

Linearity

Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric)

Scientific Data Images

Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric)

ELISA: Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric) [NBP2-81154] -

ELISA: Human Muscarinic Acetylcholine Receptor M1/CHRM1 ELISA Kit (Colorimetric) [NBP2-81154] - Standard Curve Reference

Kit Contents

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CHRM1

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.

Long Name

Cholinergic Receptor Muscarinic 1

Alternate Names

HM1, M1, M1R, mAChR M1

Gene Symbol

CHRM1

Additional CHRM1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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