Human Neprilysin/CD10 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62866

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all Neprilysin/CD10 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human NEP in samples. No significant cross-reactivity or interference between Human NEP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.41%

Inter-Assay Precision (Precision between assays) CV% < 4.52%

Recovery for Human Neprilysin/CD10 ELISA Kit (Colorimetric)

Recovery

94-112%

Linearity

Human Neprilysin/CD10 ELISA Kit (Colorimetric)

Scientific Data Images for Human Neprilysin/CD10 ELISA Kit (Colorimetric)

Human Neprilysin/CD10 ELISA Kit (Colorimetric)

ELISA: Human Neprilysin/CD10 ELISA Kit (Colorimetric) [NBP2-62866] -

ELISA: Human Neprilysin/CD10 ELISA Kit (Colorimetric) [NBP2-62866] - Standard Curve Reference

Kit Contents for Human Neprilysin/CD10 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Neprilysin/CD10

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Gene Symbol

MME

Additional Neprilysin/CD10 Products

Product Documents for Human Neprilysin/CD10 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Neprilysin/CD10 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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