Human Nociceptin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81196

Novus Biologicals, part of Bio-Techne
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NBP2-81196
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

2.81 pg/mL

Assay Range

4.69 - 300 pg/mL

View all Nociceptin ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Nociceptin in samples. No significant cross-reactivity or interference between Human Nociceptin and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.23%

Inter-Assay Precision (Precision between assays) CV% < 4.67%

Recovery for Human Nociceptin ELISA Kit (Colorimetric)

Recovery

88-107%

Linearity

Human Nociceptin ELISA Kit (Colorimetric)

Scientific Data Images for Human Nociceptin ELISA Kit (Colorimetric)

Human Nociceptin ELISA Kit (Colorimetric)

ELISA: Human Nociceptin ELISA Kit (Colorimetric) [NBP2-81196] -

ELISA: Human Nociceptin ELISA Kit (Colorimetric) [NBP2-81196] - Standard Curve Reference

Kit Contents for Human Nociceptin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Nociceptin

Nociceptin (Orphanin FQ, OFQ), a heptadecapeptide peptide, has been designated as an endogenous ligand for the ORL1 receptor. The amino acid sequence of nociceptin hashomology with other opioid peptides, especially the prodynorphin fragment dynorphin A, suggesting a close evolutionary relationship between the precursors. A diffuse distribution throughout the brain is seen with binding studies and in situ hybridization suggesting an extensive role for nociceptin in a multitude of CNS functions. Immunocytochemical localizations in rat spinal cord have demonstrated nociceptin abundance in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal. Nociceptin immunoreactivity was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. The localization is supported by its function in processing of nociceptive signals.

Alternate Names

nociceptin, nocistatin, OFQ, P, PPNOC, prepronociceptin, propronociceptin

Gene Symbol

PNOC

Additional Nociceptin Products

Product Documents for Human Nociceptin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Nociceptin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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