Norepinephrine ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA3836

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Biological Fluid (1-750 uL)

Sensitivity

0.1 ng/mL x C*
C* = Correction factor

Assay Range

0.2 to 32 ng/mL

View all Norepinephrine ELISA Kits »

Product Specifications

Assay Type

Quantification

Kit Type

ELISA Kit (Colorimetric)

Description

Norepinephrine ELISA Kit is an enzyme immunoassay for the quantitative determination of Norepinephrine in biological fluids.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 15.6 %

Recovery for Norepinephrine ELISA Kit (Colorimetric)

Recovery

Human EDTA-Plasma: 116.5%, Cell Culture Medium: 96.7%

Kit Contents for Norepinephrine ELISA Kit (Colorimetric)

  • Acylation Reagent
  • Adhesive Foil
  • Adjustment Buffer
  • Coenzyme
  • Enzyme
  • Enzyme Conjugate
  • Extraction Plate
  • Hydrochloric Acid
  • Microtiter Plate
  • Noradrenaline Antiserum
  • Noradrenaline Microtiter Strips
  • Stop Solution
  • Substrate
  • TE Buffer
  • Wash Buffer Concentrate (50X)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Norepinephrine

Noradrenaline (Norepinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.

Alternate Names

Arterenol, Levarterenol, L-Noradrenaline, L-Norepinephrine, noradrenaline

Additional Norepinephrine Products

Product Documents for Norepinephrine ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Norepinephrine ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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