Rat Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80236

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

0.094 ng/mL

Assay Range

0.156-10 ng/mL

View all Orexin B/Hypocretin-2 ELISA Kits »

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat OXB in samples. No significant cross-reactivity or interference between Rat OXB and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.61%

Inter-Assay Precision (Precision between assays) CV% < 4.67%

Recovery for Rat Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)

Recovery

86-108%

Linearity

Rat Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)

Rat Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)

ELISA: Rat Orexin B/Hypocretin-2 ELISA Kit (Colorimetric) [NBP2-80236] -

ELISA: Rat Orexin B/Hypocretin-2 ELISA Kit (Colorimetric) [NBP2-80236] - Standard Curve Reference

Kit Contents for Rat Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Orexin B/Hypocretin-2

Orexin A and orexin B are neuropeptide hormones derived from proteolytic processing of the same orexin precursor protein. Orexins are named for their orexigenic-inducing (orexis; Greek for appetite) activity. They are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism. Orexins A and B are less than 50% amino acid identical. Orexin A is a ligand for two 7-transmembrane orexin receptors, OX1R and OX2R, while orexin B preferentially binds OX2R. Defects in the orexin precursor are a cause of narcolepsy in humans.

Alternate Names

Hcrt2, Hypocretin-2, Hypocretin2, PPORX, PPOX

Gene Symbol

HCRT

Additional Orexin B/Hypocretin-2 Products

Product Documents for Rat Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Orexin B/Hypocretin-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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