I am interested in your Rat Osteocalcin ELISA Kit (Colorimetric), Cat#NBP2-68153. Could you please tell us whether there are the components containing recombinant protein in this kit? If yes, could you tell us the expression systems of the recombinant protein? For Example, E.coli, Yeast, HEK293 cells, CHO cells and insect cells etc?

Name: Rat Osteocalcin ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-68153

The reference standard is a recombinant Rat mature Osteocalcin (50~99aa) proitein which was experimentally expressed using E.coli host.

Rat Osteocalcin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68153

Submit a review

Citations (5)

Product Datasheet / COA / SDS / Protocols & Manuals

100% Guarantee

Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78-50 ng/mL

View all Osteocalcin ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat OC/BGP in samples. No significant cross-reactivity or interference between Rat OC/BGP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.02%

Inter-Assay Precision (Precision between assays) CV% < 5.33%

Recovery for Rat Osteocalcin ELISA Kit (Colorimetric)

Recovery

85-109%

Linearity

Scientific Data Images for Rat Osteocalcin ELISA Kit (Colorimetric)

ELISA: Rat Osteocalcin ELISA Kit (Colorimetric) [NBP2-68153] -

ELISA: Rat Osteocalcin ELISA Kit (Colorimetric) [NBP2-68153] - Standard Curve Reference

Kit Contents for Rat Osteocalcin ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Osteocalcin

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium

Long Name

Bone gamma-Carboxyglutamate [gla] Protein

Alternate Names

BGLAP, BGP, OCN

Gene Symbol

BGLAP

Additional Osteocalcin Products

Product Documents for Rat Osteocalcin ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Rat Osteocalcin ELISA Kit (Colorimetric) Manual

COA

SDS

Download SDS

Product Specific Notices for Rat Osteocalcin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations
Reviews

Protocols
FAQs