Rat p16INK4a/CDKN2A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75211

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

View all p16INK4a / CDKN2A ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CDKN2A (p16-INK4a, p16 isoform) in samples. No significant cross-reactivity or interference between Rat CDKN2A and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.7%

Inter-Assay Precision (Precision between assays) CV% < 5.01%

Recovery for Rat p16INK4a/CDKN2A ELISA Kit (Colorimetric)

Recovery

88-109%

Linearity

Rat p16INK4a/CDKN2A ELISA Kit (Colorimetric)

Scientific Data Images for Rat p16INK4a/CDKN2A ELISA Kit (Colorimetric)

Rat p16INK4a/CDKN2A ELISA Kit (Colorimetric)

ELISA: Rat p16INK4a/CDKN2A ELISA Kit (Colorimetric) [NBP2-75211] -

ELISA: Rat p16INK4a/CDKN2A ELISA Kit (Colorimetric) [NBP2-75211] - Standard Curve Reference

Kit Contents for Rat p16INK4a/CDKN2A ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: p16INK4a / CDKN2A

CDKN2A/p16INK4a is a gene that codes for a widely expressed protein with four isoforms, measuring 156, 105, 116, and 167 amino acids in length with weights of approximately 17, 11, 12, and 18 kDa respectively. CDKN2A/p16INK4a functions as a negative regulator of the proliferation of cells, which then allows them to interact with cyclins D and to phosphorylate the retinoblastoma protein. Current studies are being done on several diseases and disorders related to this gene including Li-Fraumeni syndrome, malignant peripheral nerve sheath tumor, blastic plasmacytoid dendritic cell, acth-secreting pituitary adenoma, recessive dystrophic epidermolysis bullosa, marginal zone b-cell lymphoma, adult astrocytic tumor, melanoma, and squamous cell carcinoma. CDKN2A/p16INK4a has also been shown to have interactions with MDM2, ZNF420, EGR1, MYCN, and CDK6 in pathways such as the cell cycle, p53 signaling, mitochondrial apoptosis, HIF-1 Alpha, CRHR, pancreatic cancer, and glioma pathways.

Long Name

p16 Cyclin Dependent Kinase 4 Inhibitor 2A

Alternate Names

ARF, CDK4I, CDKN2A, INK4a, MLM, p14ARF, p16, p19ARF

Gene Symbol

CDKN2A

Additional p16INK4a / CDKN2A Products

Product Documents for Rat p16INK4a/CDKN2A ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat p16INK4a/CDKN2A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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