Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75207

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

View all p21/CIP1/CDKN1A ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CDKN1A in samples. No significant cross-reactivity or interference between Rat CDKN1A and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5%

Inter-Assay Precision (Precision between assays) CV% < 4.92%

Recovery for Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

Recovery

90-107%

Linearity

Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

Scientific Data Images for Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

ELISA: Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric) [NBP2-75207] -

ELISA: Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric) [NBP2-75207] - Standard Curve Reference

Kit Contents for Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: p21/CIP1/CDKN1A

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Gene Symbol

CDKN1A

Additional p21/CIP1/CDKN1A Products

Product Documents for Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat p21/CIP1/CDKN1A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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