Human p53 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75355

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63-1000 pg/mL

View all p53 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human TP53 in samples. No significant cross-reactivity or interference between Human TP53 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10%

Inter-Assay Precision (Precision between assays) CV% < 11.73%

Recovery for Human p53 ELISA Kit (Chemiluminescence)

Recovery

85-108%

Linearity

Human p53 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human p53 ELISA Kit (Chemiluminescence)

Human p53 ELISA Kit (Chemiluminescence)

ELISA: Human p53 ELISA Kit (Chemiluminescence) [NBP2-75355] -

ELISA: Human p53 ELISA Kit (Chemiluminescence) [NBP2-75355] - Standard Curve Reference

Kit Contents for Human p53 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for Human p53 ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human p53 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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