Human PARP ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18217
Key Product Details
Sample Type & Volume Required Per Well
Cell lysates, tissue homogenates and other biological fluids (100 ul)
Sensitivity
0.33 ng/ml
Assay Range
0.78-50 ng/ml
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human PARP in the sample, this Human PARP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human PARP concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10
Inter-Assay Precision (Precision between assays) %CV < 15
Scientific Data Images for Human PARP ELISA Kit (Colorimetric)
![ELISA: Human PARP ELISA Kit (Colorimetric) [NBP3-18217]](https://resources.bio-techne.com/images/products/Human-PARP-ELISA-Kit-Colorimetric-ELISA-NBP3-18217-img0001.jpg "ELISA: Human PARP ELISA Kit (Colorimetric) [NBP3-18217]")
ELISA: Human PARP ELISA Kit (Colorimetric) [NBP3-18217]
ELISA: Human PARP ELISA Kit (Colorimetric) [NBP3-18217] - Standard Curve ReferenceKit Contents for Human PARP ELISA Kit (Colorimetric)
- Balance Solution
- Enzyme Conjugate
- Microtiter Plate
- Standards
- Stop Solution
- Substrate A
- Substrate B
- Wash Solution
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: PARP
PARP is inactivated by caspase cleavage, leading to a programmed cell death pathway.
Long Name
Poly [ADP-ribose] Polymerase
Alternate Names
ADPRT, PARP1, PPOL
Gene Symbol
PARP1
Additional PARP Products
Product Documents for Human PARP ELISA Kit (Colorimetric)
Product Specific Notices for Human PARP ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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