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Mouse PLA2G1B ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80372

Novus Biologicals, part of Bio-Techne
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NBP2-80372

Key Product Details

Sample Type & Volume Required Per Well

Serum (100 ul)

Sensitivity

0.66 pg/ml

Assay Range

2.82-180 pg/ml (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Mouse

Specificity

Recognizes both recombinant and natural Mouse PLA2G1B.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.8

Inter-Assay Precision (Precision between assays) CV% < 6.9 %

Recovery for Mouse PLA2G1B ELISA Kit (Colorimetric)

Recovery

88-91%

Scientific Data Images for Mouse PLA2G1B ELISA Kit (Colorimetric)

ELISA: Mouse PLA2G1B ELISA Kit (Colorimetric) [NBP2-80372]

ELISA: Mouse PLA2G1B ELISA Kit (Colorimetric) [NBP2-80372]

ELISA: Mouse PLA2G1B ELISA Kit (Colorimetric) [NBP2-80372] - Example standard curve for Mouse PLA2G1B ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Mouse PLA2G1B ELISA Kit (Colorimetric)

  • 1. 96 well microplate coated with Capture Antibody
  • 2. Detection Antibody conjugated to HRP
  • 3. Standards
  • 4. Wash Buffer Concentrate
  • 5. Dilution Buffer Concentrate
  • 6. Color Reagent A
  • 7. Color Reagent B
  • 8. Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: PLA2G1B

Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM]

Long Name

Phospholipase A2 Group IB

Alternate Names

PLA2, PLA2A, PPLA2

Gene Symbol

PLA2G1B

Additional PLA2G1B Products

Product Documents for Mouse PLA2G1B ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse PLA2G1B ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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