Human Prolactin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60128

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell culture supernates, tissue homogenates and cell lysates (50 - 100 uL)

Sensitivity

50 uIU/mL

Assay Range

40 uIU/mL - 2000 uIU/mL

View all Prolactin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human Prolactin. No significant cross-reactivity or interference between human Prolactin and analogues was observed

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Scientific Data Images for Human Prolactin ELISA Kit (Colorimetric)

ELISA: Human Prolactin ELISA Kit (Colorimetric) [NBP2-60128]

ELISA: Human Prolactin ELISA Kit (Colorimetric) [NBP2-60128]

ELISA: Human Prolactin ELISA Kit (Colorimetric) [NBP2-60128] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human Prolactin ELISA Kit (Colorimetric)

Human Prolactin ELISA Kit (Colorimetric) [NBP2-60128] -

Human Prolactin ELISA Kit (Colorimetric) [NBP2-60128] - Standard Curve Reference

Kit Contents for Human Prolactin ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Prolactin

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for PRL. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

PRL

Gene Symbol

PRL

Additional Prolactin Products

Product Documents for Human Prolactin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Prolactin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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