Human Prothrombin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60590

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell Culture, Urine, Saliva, Milk, CSF

Sensitivity

0.2 ng/ml (example only; lot dependent)

Assay Range

0.344 - 22 ng/mL (example only; lot dependent)

View all Prothrombin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

Human prothrombin

Description

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.80% (example only; lot dependent)

Inter-Assay Precision (Precision between assays) CV% < 8.80% (example only; lot dependent)

Recovery for Human Prothrombin ELISA Kit (Colorimetric)

Recovery

100.00% (example only; lot dependent)

Scientific Data Images for Human Prothrombin ELISA Kit (Colorimetric)

ELISA: Human Prothrombin ELISA Kit (Colorimetric) [NBP2-60590]

ELISA: Human Prothrombin ELISA Kit (Colorimetric) [NBP2-60590]

ELISA: Human Prothrombin ELISA Kit (Colorimetric) [NBP2-60590] - Standard Curve.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: Prothrombin

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) at residue 44 leads to formation of Thrombin in the first step of the coagulation cascade. Thrombin cleaves bonds between Arg and Gly and activates Factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.

Alternate Names

Coagulation factor II, coagulation factor II (thrombin), EC 3.4.21, EC 3.4.21.5, prothrombin, prothrombin B-chain, PT, serine protease

Gene Symbol

F2

Additional Prothrombin Products

Product Documents for Human Prothrombin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Prothrombin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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