Human PSGL-1/CD162 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91256
Key Product Details
Sample Type & Volume Required Per Well
Cell culture supernatant, serum (100 uL (1:20 prediluted))
Sensitivity
1.0 U/mL
Assay Range
1.6 - 50 U/mL
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human PSGL-1 positive serum. There was no crossreactivity detected.
Description
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 3.20%
Inter-Assay Precision (Precision between assays) CV% < 6.60%
Recovery for Human PSGL-1/CD162 ELISA Kit (Colorimetric)
Recovery
The amount of endogenous human PSGL-1 in unspiked serum was subtracted from the spike values. The recovery ranged from 83% to 104% with an overall mean recovery of 94%.
Scientific Data Images for Human PSGL-1/CD162 ELISA Kit (Colorimetric)
![ELISA: Human PSGL-1/CD162 ELISA Kit (Colorimetric) [NBP1-91256]](https://resources.bio-techne.com/images/products/PSGL-1-CD162-ELISA-Kit-ELISA-NBP1-91256-img0001.jpg "ELISA: Human PSGL-1/CD162 ELISA Kit (Colorimetric) [NBP1-91256]")
ELISA: Human PSGL-1/CD162 ELISA Kit (Colorimetric) [NBP1-91256]
ELISA: PSGL-1/CD162 ELISA Kit [NBP1-91256] - Standard curveKit Contents for Human PSGL-1/CD162 ELISA Kit (Colorimetric)
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human PSGL-1
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human PSGL-1 monoclonal antibody
- Human PSGL-1 Standard lyophilized, 100 U/mL upon reconstitution
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: PSGL-1/CD162
Long Name
P-Selectin Glycoprotein Ligand 1
Alternate Names
CD162, PSGL1, SELPLG
Gene Symbol
SELPLG
Additional PSGL-1/CD162 Products
Product Documents for Human PSGL-1/CD162 ELISA Kit (Colorimetric)
Product Specific Notices for Human PSGL-1/CD162 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Loading...
Loading...
Loading...
Loading...