Human PSP94/MSMB ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08154

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Biological fluids

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all PSP94/MSMB ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.66

Inter-Assay Precision (Precision between assays) %CV < 5.00

Recovery for Human PSP94/MSMB ELISA Kit (Colorimetric)

Recovery

86 - 106%

Linearity

Human PSP94/MSMB ELISA Kit (Colorimetric)

Scientific Data Images for Human PSP94/MSMB ELISA Kit (Colorimetric)

Human PSP94/MSMB ELISA Kit (Colorimetric)

ELISA: Human PSP94/MSMB ELISA Kit (Colorimetric) [NBP3-08154] -

ELISA: Human PSP94/MSMB ELISA Kit (Colorimetric) [NBP3-08154] - Standard Curve Reference

Kit Contents for Human PSP94/MSMB ELISA Kit (Colorimetric)

  • Micro ELISA Plate (Dismountable) - 1 EA, Reference Standard - 2 vials, Concentrated Biotinylated Detection Ab (100X) - 120 uL, Concentrated HRP Conjugate (100X) - 120 uL, Reference Standard & Sample Diluent - 20 mL, Biotinylated Detection Ab Diluent - 14

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PSP94/MSMB

PSP94 (prostate secretory protein of 94 amino acids; also named beta-MSP) is a secreted, non-glycosylated member of the beta-microseminoprotein family. The 94 amino acid (aa) mature PSP94 contains no classic motifs or domains, but does have ten Cys that are conserved across species. It is expressed in mucoid secretions, but its function is unknown. PSP94 is abundant in prostatic fluid, which is the exclusive source in rodents. Gastric and respiratory secretory epithelia are also significant sources in humans. Human PSP94 circulates bound to a 71 kDa PSP binding protein, possibly disulfide-linked. The seminal fluid protein CRISP-3 can also bind PSP94. PSP94 has been proposed as an alternative to PSA as a serum marker for prostate cancer. When total (bound plus free) PSP94 is considered, its secretion is found to be down-regulated in cancer cells, creating below normal circulating levels. Its size is predicted at 11 kDa, but may appear to be 16 kDa due to anomalous migration. A 61 aa variant, formed by C-terminal proteolysis, is increased in prostate secretions from patients with benign prostatic hyperplasia. A prostate-specific alternate splice form shows a substitution of 41 aa for the C-terminal 78 aa. Mature human PSP94 shares 53%, 46%, 43%, and 42% aa identity with porcine, rat, mouse, and chicken PSP94, respectively. Most of the ten primate sequences available show less than 80% aa identity. PSP94 has been proposed as a species barrier protein due to its low evolutionary conservation and abundance in seminal fluid.

Long Name

Prostate Secretory Protein of 94 Amino Acids/Microseminoprotein, beta

Alternate Names

IGBF, MSMB, MSPB, PIP, PN44, PRPS, PSP57

Gene Symbol

MSMB

Additional PSP94/MSMB Products

Product Documents for Human PSP94/MSMB ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human PSP94/MSMB ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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