Human Pyruvate Dehydrogenase E1 beta subunit ELISA Kit (Colorimetric)

Name: Human Pyruvate Dehydrogenase E1 beta subunit ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-40298

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40298

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)

Sensitivity

1.2 U/L (example only; lot dependent)

Assay Range

1.56 - 100 U/L (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Pyruvate Dehydrogenase E1 beta subunit ELISA Kit (Colorimetric)

ELISA: Human Pyruvate Dehydrogenase E1 beta subunit ELISA Kit (Colorimetric) [NBP3-40298]

ELISA: Human Pyruvate Dehydrogenase E1 beta subunit ELISA Kit (Colorimetric) [NBP3-40298] - Standard Curve Reference

Kit Contents for Human Pyruvate Dehydrogenase E1 beta subunit ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Pyruvate Dehydrogenase E1 beta subunit

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)

Alternate Names

DKFZp564K0164, mitochondrial, pyruvate dehydrogenase (lipoamide) beta, pyruvate dehydrogenase, E1 beta polypeptide

Gene Symbol

PDHB

Additional Pyruvate Dehydrogenase E1 beta subunit Products

Product Documents for Human Pyruvate Dehydrogenase E1 beta subunit ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Pyruvate Dehydrogenase E1 beta subunit ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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