Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40299

Novus Biologicals, part of Bio-Techne
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NBP3-40299
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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)

Sensitivity

1.2 U/L (example only; lot dependent)

Assay Range

1.56 - 100 U/L (example only; lot dependent)

View all Pyruvate Dehydrogenase E1 beta subunit ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images

Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40299]

ELISA: Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40299] - Standard Curve Reference

Kit Contents

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Pyruvate Dehydrogenase E1 beta subunit

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)

Alternate Names

DKFZp564K0164, mitochondrial, pyruvate dehydrogenase (lipoamide) beta, pyruvate dehydrogenase, E1 beta polypeptide

Gene Symbol

PDHB

Additional Pyruvate Dehydrogenase E1 beta subunit Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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