Human RANK/TNFRSF11A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1255

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant and Serum (100 uL)

Sensitivity

2 pg/mL

Assay Range

62.5 to 4000 pg/mL

View all RANK/TNFRSF11A ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

TNFRSF11A (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human TNFRSF11A.

Kit Contents for Human RANK/TNFRSF11A ELISA Kit (Colorimetric)

  • 96-well plate precoated with anti- human RANK antibody
  • ABC diluent buffer
  • Adhesive cover
  • Antibody diluent buffer
  • Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  • Biotinylated anti- human RANK antibody, dilution 1:100
  • Lyophilized recombinant human RANK standard
  • Sample diluent buffer
  • TMB color developing agent
  • TMB stop solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RANK/TNFRSF11A

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family (1). RANK ligand (RANKL/TRANCE/ OPGL) (mAbs to RANKL is also available from Imgenex, Cat # IMG-133) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4). Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways (3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2 (4).

Long Name

Receptor Activator of NF-kappa-B

Alternate Names

CD265, ODFR, TNFRSF11A, TRANCE R

Gene Symbol

TNFRSF11A

Additional RANK/TNFRSF11A Products

Product Documents for Human RANK/TNFRSF11A ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human RANK/TNFRSF11A ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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