Rat ICAM-1/CD54 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # RIC100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
Sensitivity
4.1 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Product Summary for Rat ICAM-1/CD54 Quantikine ELISA Kit
The Quantikine Rat sICAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat sICAM-1 levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant rat sICAM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring rat sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural rat sICAM-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Rat
Specificity
Natural and recombinant rat sICAM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 47 | 39 | 47 |
| Mean (pg/mL) | 87 | 271 | 617 | 80 | 307 | 560 |
| Standard Deviation | 4.3 | 13 | 29 | 7.6 | 19 | 35 |
| CV% | 4.9 | 4.8 | 4.7 | 9.5 | 6.2 | 6.2 |
Recovery for Rat ICAM-1/CD54 Quantikine ELISA Kit
The recovery of rat sICAM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=8) | 99 | 88-104 |
| EDTA Plasma (n=5) | 103 | 91-117 |
| Serum (n=6) | 103 | 83-119 |
Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of rat sICAM-1 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Rat ICAM-1/CD54 Quantikine ELISA Kit
Rat ICAM-1/CD54 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ICAM-1/CD54
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
Additional ICAM-1/CD54 Products
Product Documents for Rat ICAM-1/CD54 Quantikine ELISA Kit
Product Specific Notices for Rat ICAM-1/CD54 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.