Rat/Mouse Cytochrome c Quantikine ELISA Kit

Name: Rat/Mouse Cytochrome c Quantikine ELISA Kit
Brand: R&D Systems
SKU: MCTC0
Rating: 5 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # MCTC0

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

2.5 hours

Sample Type & Volume Required Per Well

Cell Lysates (50 µL), Subcellular Fractions (50 µL)

Sensitivity

0.5 ng/mL

Assay Range

0.78-25 ng/mL (Cell Lysates, Subcellular Fractions)

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Product Summary for Rat/Mouse Cytochrome c Quantikine ELISA Kit

The Quantikine Rat/Mouse Cytochrome c Immunoassay is a 2.5 hour solid phase ELISA designed to measure rat and mouse cytochrome c. It contains natural rat cytochrome c and antibodies raised against cytochrome c, and because of the 100% sequence identity of rat and mouse cytochrome c, this kit can be used to accurately quantitate natural rat or mouse cytochrome c in cell lysates and subcellular fractions.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Mouse, Rat

Specificity

This kit recognizes both the reduced and oxidized forms of natural rat and mouse cytochrome c.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Lysates, Subcellular Fractions

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	1.61	8.82	13.4	1.72	8.91	13.5
Standard Deviation	0.02	0.09	0.19	0.06	0.31	0.59
CV%	1.24	1.02	1.42	3.49	3.48	4.37

Recovery for Rat/Mouse Cytochrome c Quantikine ELISA Kit

The recovery of rat/mouse Cytochrome c spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
PBS/0.01% Saponin (n=2)	94	90-98
PBS/0.05% Tween 20 (n=2)	101	100-102
PBS/0.5% Triton X-100 (n=2)	100	96-106
PBS/1% Triton X-100 (n=2)	98	95-101

Linearity

To assess the linearity of the assay, five or more samples spiked with various concentrations of rat/mouse Cytochrome c in each matrix were diluted with Calibrator Diluent and then assayed.

Scientific Data Images for Rat/Mouse Cytochrome c Quantikine ELISA Kit

Mouse/Rat Cytochrome c ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cytochrome c

Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.

Alternate Names

CYCS

Entrez Gene IDs

54205 (Human)

Gene Symbol