Rat TIMP-1 Quantikine ELISA Kit

Name: Rat TIMP-1 Quantikine ELISA Kit
Brand: R&D Systems
SKU: RTM100

R&D Systems, part of Bio-Techne | Catalog # RTM100

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Product Datasheet / COA / SDS

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)

Sensitivity

4.5 pg/mL

Assay Range

37.5-2400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

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Product Summary for Rat TIMP-1 Quantikine ELISA Kit

The Quantikine Rat TIMP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure rat TIMP-1 levels in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat TIMP-1 and antibodies raised against the recombinant protein. Natural rat TIMP-1 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural rat TIMP-1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Rat

Specificity

Natural and recombinant rat TIMP-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	122	443	1307	132	445	1277
Standard Deviation	4.5	14	34	9.6	27	85
CV%	3.7	3.2	2.6	7.3	6.1	6.7

Recovery for Rat TIMP-1 Quantikine ELISA Kit

The recovery of rat TIMP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	106	93-119
EDTA Plasma (n=7)	99	82-115
Serum (n=7)	98	84-120

Linearity

To assess the linearity of the assay, samples containing and/or spiked with rat TIMP-1 were diluted with Calibrator Diluent and then assayed.

Scientific Data Images for Rat TIMP-1 Quantikine ELISA Kit

Rat TIMP-1 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIMP-1

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Gene Symbol