Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # RRP300
Key Product Details
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
Sensitivity
0.023 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit
The Quantikine Rat Total Adiponectin Immunoassay is a 2.5 hour solid phase ELISA designed to measure rat Adiponectin levels in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains NS0-expressed recombinant rat Adiponectin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat Adiponectin accurately. Results obtained using natural rat Adiponectin showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards.These results indicate that this kit can be used to determine relative mass values for natural rat Adiponectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Rat
Specificity
Natural and recombinant rat Adiponectin. This assay recognizes low, medium, and high molecular weight recombinant rat Adiponectin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.587 | 1.14 | 3.42 | 0.595 | 1.19 | 3.48 |
| Standard Deviation | 0.021 | 0.025 | 0.112 | 0.056 | 0.083 | 0.272 |
| CV% | 3.6 | 2.2 | 3.3 | 9.4 | 7.0 | 7.8 |
Recovery for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit
The recovery of rat Adiponectin spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=6) | 109 | 100-116 |
| Urine (n=4) | 106 | 91-113 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of rat Adiponectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit
Rat Total Adiponectin/Acrp30 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Adiponectin/Acrp30
Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.
Alternate Names
Acrp30, AdipoQ, ApM1, GBP28
Gene Symbol
ADIPOQ
Additional Adiponectin/Acrp30 Products
Product Documents for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit
Product Specific Notices for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.