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Rat Reg1A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82146

Novus Biologicals, part of Bio-Techne
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NBP2-82146

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Reg1A in samples. No Significant cross-reactivity or interference between Rat Reg1A and analogues was observed

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Rat Reg1A ELISA Kit (Colorimetric)

Recovery

average = 94% for serum; average = 96% for EDTA plasma; average = 93% for cell culture media

Linearity

Rat Reg1A ELISA Kit (Colorimetric)

Scientific Data Images for Rat Reg1A ELISA Kit (Colorimetric)

Rat Reg1A ELISA Kit (Colorimetric)

ELISA: Rat Reg1A ELISA Kit (Colorimetric) [NBP2-82146] -

ELISA: Rat Reg1A ELISA Kit (Colorimetric) [NBP2-82146] - Standard Curve Reference

Kit Contents for Rat Reg1A ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Reg1A

The Reg family constitutes a subset of the calcium-dependent C-type lectin superfamily. These small, secreted proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. Studies also indicate a role for Reg family members in tumor formation and indicate their potential for use as biomarkers of carcinogenesis.

Reg1A, also known as PTP, PSP, and lithostathine, is a member of the Reg family of secreted proteins with a C-type lectin domain. Due to variable glycosylation, pancreatic Reg1A exists as multiple species of 16 - 18 kDa. Reg1A promotes the maintenance and growth of pancreatic islet β-cells and intestinal villi. It is upregulated in pancreatitis and some carcinomas. Reg1A is an antigenic target in autoimmune diabetes. Human Reg1A shares 65% - 68% aa sequence identity with mouse and rat Reg1A.

Long Name

Regenerating Islet-derived 1A

Alternate Names

ICRF, PSP, PTP

Gene Symbol

REG1A

Additional Reg1A Products

Product Documents for Rat Reg1A ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Reg1A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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