Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75054

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

3.75 ng/mL

Assay Range

6.25 - 400 ng/mL

View all Serpin A6 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CBG in samples. No significant cross-reactivity or interference between Rat CBG and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.25%

Inter-Assay Precision (Precision between assays) CV% < 4.39%

Recovery for Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric)

Recovery

89-108%

Linearity

Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric)

Scientific Data Images for Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric)

Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric)

ELISA: Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric) [NBP2-75054] -

ELISA: Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric) [NBP2-75054] - Standard Curve Reference

Kit Contents for Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Serpin A6

Corticol binding globulin (CBG or transcotin) binds with high affinity to cortisol in plasma. Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Alternate Names

CBG

Gene Symbol

SERPINA6

Additional Serpin A6 Products

Product Documents for Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Serpin A6/Cortisol Binding Globulin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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