Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80301

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

0.094 ng/mL

Assay Range

0.156-10 ng/mL

View all Sirtuin 1/SIRT1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse SIRT1 in samples. No significant cross-reactivity or interference between Mouse SIRT1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.76%

Inter-Assay Precision (Precision between assays) CV% < 5.99%

Recovery for Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

Recovery

83-109%

Linearity

Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

ELISA: Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric) [NBP2-80301] -

ELISA: Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric) [NBP2-80301] - Standard Curve Reference

Kit Contents for Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Sirtuin 1/SIRT1

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Gene Symbol

SIRT1

UniProt

Q923E4 (Mouse)

Additional Sirtuin 1/SIRT1 Products

Product Documents for Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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