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Rat SMPD1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69874

Novus Biologicals, part of Bio-Techne
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NBP2-69874

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat ASM in samples. No significant cross-reactivity or interference between Rat ASM and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.79%

Inter-Assay Precision (Precision between assays) CV% < 4.87%

Recovery for Rat SMPD1 ELISA Kit (Colorimetric)

Recovery

84-110%

Linearity

Rat SMPD1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat SMPD1 ELISA Kit (Colorimetric)

Rat SMPD1 ELISA Kit (Colorimetric)

ELISA: Rat SMPD1 ELISA Kit (Colorimetric) [NBP2-69874] -

ELISA: Rat SMPD1 ELISA Kit (Colorimetric) [NBP2-69874] - Standard Curve Reference

Kit Contents for Rat SMPD1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SMPD1

The protein encoded by the SMPD1 gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Three transcript variants encoding two different isoforms have been found for this gene. (provided by RefSeq)

Long Name

Sphingomyelin Phosphodiesterase 1, Acid Lysosomal

Alternate Names

aSMase, NPD

Gene Symbol

SMPD1

Additional SMPD1 Products

Product Documents for Rat SMPD1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat SMPD1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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