Fish SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21154

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.625 ng/mL

Assay Range

1.25 ng/mL - 20 ng/mL

View all SOD1/Cu-Zn SOD ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Fish

Specificity

This assay has high sensitivity and excellent specificity for detection of fish SOD1/Cu-Zn SOD. No significant cross-reactivity or interference between fish SOD1/Cu-Zn SOD and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Recovery for Fish SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

Recovery

85 - 103%

Scientific Data Images for Fish SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

Fish SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

Fish SOD1/Cu-Zn SOD ELISA Kit (Colorimetric) [NBP3-21154] -

Fish SOD1/Cu-Zn SOD ELISA Kit (Colorimetric) [NBP3-21154] - Standard Curve Reference

Kit Contents for Fish SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (10 x concentrate)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SOD1/Cu-Zn SOD

FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Gene Symbol

SOD1

Additional SOD1/Cu-Zn SOD Products

Product Documents for Fish SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fish SOD1/Cu-Zn SOD ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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