Mouse SPARC ELISA Kit (Chemiluminescence)

Name: Mouse SPARC ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP2-68074

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68074

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.38 ng/mL

Assay Range

0.63-40 ng/mL

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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Mouse

Specificity

This kit recognizes Mouse ON in samples. No significant cross-reactivity or interference between Mouse ON and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 9.24%

Inter-Assay Precision (Precision between assays) CV% < 10.19%

Recovery for Mouse SPARC ELISA Kit (Chemiluminescence)

Recovery

85-110%

Linearity

Scientific Data Images for Mouse SPARC ELISA Kit (Chemiluminescence)

ELISA: Mouse SPARC ELISA Kit (Chemiluminescence) [NBP2-68074] -

ELISA: Mouse SPARC ELISA Kit (Chemiluminescence) [NBP2-68074] - Standard Curve Reference

Kit Contents for Mouse SPARC ELISA Kit (Chemiluminescence)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro CLIA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Substrate Reagent A
Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SPARC

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Alternate Names

BM-40, Osteonectin

Gene Symbol

SPARC

Additional SPARC Products

Product Documents for Mouse SPARC ELISA Kit (Chemiluminescence)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Mouse SPARC ELISA Kit (Chemiluminescence) Manual

COA

Product Specific Notices for Mouse SPARC ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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